SEM — Smooth-surfaced spores of different sizes produced on agar plates at 55 °C (spores, not vegetative cells). Species-level image (Moorella thermoacetica ATCC 39073). Adapted from Malleck T et al. (2022), Int J Mol Sci 23(1):550, Fig. 1A. Source · CC BY 4.0 · Cropped to panel A; converted to WebP

Electrotroph · Acetogen · Homoacetogen

Moorella thermoacetica

Also known as Clostridium thermoaceticum

Bacillota · Clostridia · Thermoanaerobacteraceae

ElectroactiveEET inwardBSL-1Partial record
  • Acetogen
  • Thermophile
  • Wood ljungdahl
  • Tier 2
Inward
Electron transfer
electroactive
55°C
Optimal temperature
grows 45–65 °C
6.8
Optimal pH
grows pH 6–7.5

01 · Identity

Identity

Where this organism sits in the tree of life, how to obtain it, and what a cell looks like.

Lineage

  1. domainBacteria
  2. ›phylumBacillota
  3. ›classClostridia
  4. ›orderThermoanaerobacterales
  5. ›familyThermoanaerobacteraceae
  6. ›genusMoorella
  7. ›speciesMoorella thermoacetica

Culture collections & accessions

DSMZ
DSM 521
ATCC
ATCC 39073
NCBI taxon
1525

Cell morphology

Rod, Gram-positive (monoderm)

  • Motile: no
  • Spore-forming: yes

02 · Electron transfer

Electron transfer

How electrons cross the cell envelope — the property that makes a microbe useful in an electrochemical system.

ElectroactiveEET inward

No extracellular electron-transfer route is curated for this organism yet.

Mechanisms

Other

Cathode electron uptake mechanism
Contested

03 · Growth envelope

Growth envelope

The conditions this organism tolerates and what it eats and breathes — the operating window for a reactor.

ThermophileObligate anaerobeMixotroph
Temperature55 (45–65) °C
0100 °C
pH6.8 (6–7.5)
014

Energy metabolism

Electron donors

  • H2
  • CO
  • Cathode
  • Glucose

Electron acceptors

  • CO2
  • Nitrate

Carbon sources

  • CO2
  • H2
  • CO
  • Glucose
  • Fructose
  • Methanol

Metabolism — detail

ATP synthase type
Proton dependent
Carbon fixation pathway
Wood ljungdahl

04 · Growth & electron-transfer kinetics

Growth & electron-transfer kinetics

How fast this organism grows and how hard it pushes electrons into an anode, as the corpus reports it, and what those constants imply when you run them.

Kinetic evidence

ParameterMedian10–90 %nSource
Max. specific growth rate μmax0.1 h⁻¹0.04–0.23genus prior · 1 ref
Half-saturation constant Ks50 mg/L10–1501genus prior · 1 ref
Biomass yield Y0.06 g/g0.02–0.122genus prior · 1 ref
Decay coefficient kdNot in the corpus yet
Exchange current density i₀Not in the corpus yet
Transfer coefficient αNot in the corpus yet
Tafel slopeNot in the corpus yet

Median of the extracted values, the 10–90 % spread behind it and how many values it pools.

Evidence rows (0)

Genus-level prior: Moorella thermoacetica

A genus-level prior is a summary for the whole genus, not a measurement on this strain.

Constants that drive the simulation

Scenario — values you set, not evidence

Initial substrate1000 mg/L
Anode potential vs SHE0 V
Duration72 h
Initial attached biomass0.1 g/m²
Anode area per liquid volume100 m²/m³
Reactor mode

Growth simulation

Scenario: Initial substrate 1000 mg/L · Initial attached biomass 0.1 g/m² · Anode area per liquid volume 100 m²/m³ · Duration 72 h · Batch. Values you set, not evidence.

0.7g/m²
Peak biomass
20.4h
Time to 90 % substrate removal
Attached biomassg/m²
00.50204060h
Bulk substratemg/L
050010000204060h

No current model

The current needs Max. current density jmax, Half-saturation potential E_KA, which this source does not have.

  • Substrate-saturated growth
  • Slowing
  • Stationary

Monod growth has no lag phase; the bands mark where the net growth rate is near its maximum, falling, near zero or negative.

Simulator notes
  • decayPerH absent: no endogenous decay term, so growth is an upper bound
  • biofilmDensityKgPerM3 absent: thickness not computed (thicknessUm = null)
  • no anodePotentialVvsShe / overpotentialV given: current not computed (currentDensityAPerM2 = null)

These curves are a Monod and Nernst-Monod idealisation (after Marcus et al. 2007 and Torres et al. 2008), parameterised only by the constants listed above: each one carries its n and its 10–90 % range, and a constant with n = 1 has no spread at all. No diffusion inside the biofilm, no detachment, no coulombic-efficiency split. The scenario values are yours, not evidence.

05 · Performance

Performance

Reported electrochemical output, the role it plays in a reactor, and what it produces.

Products (2)

ProductMax titerg/LMax rateCoulombic efficiency
acetate—0.3 g/L/d—
formate———

Substrate → product (1)

  • CO2 + cathode (immobilized)yields
    • Acetate
    • Formate

    55°C, immobilized on carbon nanoparticles · 23.2× and 2.8× faster acetate/formate production at 55°C vs 25°C (Yu et al. 2017).

06 · Ecology & biofilm

Ecology & biofilm

How it lives on an electrode, who it partners with, and where it is found in nature.

Ecosystems

  • Soil
  • Engineered MES

Isolation sources

  • horse manure

07 · Applications, engineering & safety

Applications, engineering & safety

Where it has been put to work, how tractable it is to engineer, and what handling it requires.

Applications (2)

  • Microbial electrosynthesis acetate
    TRL 3/9
  • Carbon capture
    TRL 3/9

Engineering

Engineered strains publishedIndustrial relevance 4/5

Reactor compatibility

  • MES cathode

Biosafety

BSL-1
  • Pathogen: no

Modeling assets

Linked GEM: iMT

08 · Sources & data quality

Sources & data quality

Every value above traces to a citation. This is how complete and how confident the record is.

Record quality

PartialMedium confidenceNeeds reviewCurated: manual

Literature reviewed 2026-04-26 · schema v1.2.0 · updated 2026-04-28 · imported 2026-04-28

References (2)

  1. Fontaine FE, et al. (1942). A new type of glucose fermentation by Clostridium thermoaceticum n. sp. J Bacteriol 43:701-715.
  2. Yu L, et al. (2017). Microbial electrosynthesis at 55°C with Moorella thermoautotrophica. Appl Environ Microbiol.