TEM — Thin section of cells after exposure to Pt(II)Cl4²⁻; the dark surface deposits are platinum particles. Adapted from Maes S et al. (2017), PLoS ONE 12(1):e0169093, Fig. 3. Source · CC BY 4.0 · Cropped to the G. metallireducens / Pt(II)Cl4²⁻ panel; converted to WebP

Electrogen · DIET partner donor · Iron reducer

Geobacter metallireducens GS-15

Thermodesulfobacteriota · Desulfuromonadia · Geobacteraceae

ElectroactiveEET outwardType strainBSL-1Comprehensive record

Aromatic degrader
Uranium bioremediation
Tier 1
DIET donor

Outward

Electron transfer

electroactive

30°C

Optimal temperature

grows 25–35 °C

Optimal pH

grows pH 6.5–7.5

4Mb

Genome size

01 · Identity

Identity

Where this organism sits in the tree of life, how to obtain it, and what a cell looks like.

Lineage

domainBacteria
›phylumThermodesulfobacteriota
›classDesulfuromonadia
›orderGeobacterales
›familyGeobacteraceae
›genusGeobacter
›speciesGeobacter metallireducens GS-15

Formerly classified as Deltaproteobacteria.

Culture collections & accessions

DSMZ: DSM 7210
ATCC: ATCC 53774
NCBI taxon: 269799
Genome: GCF_000012585.1
Status: Type strain

Isolation

From Potomac River sediment, Maryland, USA by Lovley DR, Phillips EJP (1987).

Lovley DR, Phillips EJP (1988). Manganese inhibition of microbial iron reduction in anaerobic sediments.

Genome

4Mb

Genome size

59.5%

GC content

Complete

Assembly level

Cell morphology

Rod

Rod, Gram-negative (diderm)

Motile: yes
Pili: yes
Outer-membrane vesicles: no
Spore-forming: no

02 · Electron transfer

Electron transfer

How electrons cross the cell envelope — the property that makes a microbe useful in an electrochemical system.

ElectroactiveEET outward

Electrons flow outward: cell → electrode (exoelectrogen)

Mechanisms

Outer-membrane cytochromes: yes
Conductive pili / nanowires: yes

03 · Growth envelope

Growth envelope

The conditions this organism tolerates and what it eats and breathes — the operating window for a reactor.

MesophileObligate anaerobeChemoorganoheterotroph

Temperature30 (25–35) °C

0100 °C

pH7 (6.5–7.5)

014

Energy metabolism

Electron donors

Acetate
Ethanol
aromatic compounds

Electron acceptors

Fe(III)
Mn(IV)
U(VI)
Anode
Nitrate

Carbon sources

Acetate
Ethanol
Benzoate
Toluene
Phenol
p-cresol
Butyrate
Propionate

Respiratory: yes
Fermentative: no

Metabolism — detail

ATP synthase type: Proton dependent
Energy conservation mechanism: Oxidative phosphorylation

04 · Growth & electron-transfer kinetics

Growth & electron-transfer kinetics

How fast this organism grows and how hard it pushes electrons into an anode, as the corpus reports it, and what those constants imply when you run them.

Kinetic evidence

Parameter	Median	10–90 %	n	Source
Max. specific growth rate μmax	0.06 h⁻¹	0.03–0.12	3	genus prior · 2 refs
Half-saturation constant Ks	90 mg/L	20–200	2	genus prior · 2 refs
Biomass yield Y	0.07 g/g	0.04–0.1	2	genus prior · 2 refs
Decay coefficient kd	Not in the corpus yet
Exchange current density i₀	Not in the corpus yet
Transfer coefficient α	Not in the corpus yet
Tafel slope	Not in the corpus yet

Median of the extracted values, the 10–90 % spread behind it and how many values it pools.

36 rows were not pooled: their unit could not be converted unambiguously.

Evidence rows (36)

Listed, not pooled (36)

K_M0.35 mMNot verifiedgenus rowconfidence 0.93
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM = 0.28-0.42 mM (2nd cycle) || range: [0.28, 0.42] || canonical_organism: geobacter_sulfurreducens_pca || canonical_substrate: acetate”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.35 mMNot verifiedgenus rowconfidence 0.93
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM = 0.28-0.42 mM (2nd cycle) || range: [0.28, 0.42] || canonical_organism: geobacter_sulfurreducens_pca || canonical_substrate: acetate”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
vmax0.0034 mmol/h/cm²Not verifiedgenus rowconfidence 0.92
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax = 0.0031-0.0037 mmol h⁻¹ cm⁻² (2nd cycle) || range: [0.0031, 0.0037] || canonical_organism: geobacter_sulfurreducens_pca || canonical_substrate: acetate”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0034 mmol/h/cm²Not verifiedgenus rowconfidence 0.92
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax = 0.0031-0.0037 mmol h⁻¹ cm⁻² (2nd cycle) || range: [0.0031, 0.0037] || canonical_organism: geobacter_sulfurreducens_pca || canonical_substrate: acetate”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0042 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0051 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0031 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0037 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
K_M1.02 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M2.61 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.28 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.42 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
vmax0.0042 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0051 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0031 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0037 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
K_M1.02 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M2.61 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.28 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.42 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
vmax0.0042 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0051 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0031 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0037 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
K_M1.02 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M2.61 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.28 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.42 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
vmax0.0042 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0051 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0031 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
vmax0.0037 mmol Ac⁻ h⁻¹ cm⁻²Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“vmax,Ac and KM,Ac, decreased from 0.0042–0.0051 mmol Ac−h−1 cm−2 to 0.0031–0.0037 mmol Ac−h−1 cm−2”
Not used in an estimate · unrecognised kinetic parameter "vmax"
K_M1.02 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M2.61 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.28 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks
K_M0.42 mM Ac⁻Not verifiedgenus rowconfidence 0.9
Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms · 2020 · 10.1371/journal.pone.0234077
“KM,Ac, decreased from 1.02–2.61 mM Ac− to 0.28–0.42 mM Ac−”
Not used in an estimate · Michaelis-Menten Km, not Monod Ks

Genus-level prior: Geobacter metallireducens

A genus-level prior is a summary for the whole genus, not a measurement on this strain.

Constants that drive the simulation

Scenario — values you set, not evidence

Initial substrate1000 mg/L

Anode potential vs SHE0 V

Duration72 h

Initial attached biomass0.1 g/m²

Anode area per liquid volume100 m²/m³

Reactor mode

Growth simulation

Scenario: Initial substrate 1000 mg/L · Initial attached biomass 0.1 g/m² · Anode area per liquid volume 100 m²/m³ · Duration 72 h · Batch. Values you set, not evidence.

0.8g/m²

Peak biomass

38.85h

Time to 90 % substrate removal

Attached biomassg/m²

Bulk substratemg/L

No current model

The current needs Max. current density jmax, Half-saturation potential E_KA, which this source does not have.

Substrate-saturated growth
Slowing
Stationary

Monod growth has no lag phase; the bands mark where the net growth rate is near its maximum, falling, near zero or negative.

Simulator notes

decayPerH absent: no endogenous decay term, so growth is an upper bound
biofilmDensityKgPerM3 absent: thickness not computed (thicknessUm = null)
no anodePotentialVvsShe / overpotentialV given: current not computed (currentDensityAPerM2 = null)

These curves are a Monod and Nernst-Monod idealisation (after Marcus et al. 2007 and Torres et al. 2008), parameterised only by the constants listed above: each one carries its n and its 10–90 % range, and a constant with n = 1 has no spread at all. No diffusion inside the biofilm, no detachment, no coulombic-efficiency split. The scenario values are yours, not evidence.

05 · Performance

Performance

Reported electrochemical output, the role it plays in a reactor, and what it produces.

Strong electrogen

Role in a reactor

Anode chamberAnode: primary current producer

06 · Ecology & biofilm

Ecology & biofilm

How it lives on an electrode, who it partners with, and where it is found in nature.

Biofilm

Forms biofilm: yes

Preferred electrode materials

Graphite
carbon cloth

Electron-exchange partners (2)

gives e⁻ to →Methanosarcina acetivorans
ethanol-fed methanogenic coculture
Co culture demonstrated
gives e⁻ to →Methanothrix harundinacea
ethanol→methane stoichiometric conversion
Co culture demonstrated

Conductive-mineral mediation

Mediates via conductive minerals: yes

Magnetite

Magnetite-stimulated DIET with Methanothrix harundinacea (Liu 2015).

Ecosystems

Freshwater
Subsurface
Rice paddy

Isolation sources

freshwater sediment, Potomac River, USA

07 · Applications, engineering & safety

Applications, engineering & safety

Where it has been put to work, how tractable it is to engineer, and what handling it requires.

Applications (3)

Bioremediation organics
TRL 5/9
Bioremediation metals
TRL 5/9
Bioelectricity generation
TRL 4/9

Engineering

Engineered strains publishedIndustrial relevance 4/5

Reactor compatibility

MFC dual chamber
Smfc soil
Sediment MFC

Genetics

Genetically tractable: yes

qPCR targets

pilA
ppcA

Biosafety

BSL-1

Pathogen: no

Modeling assets

Linked GEM: iAF987.metallireducens

08 · Sources & data quality

Sources & data quality

Every value above traces to a citation. This is how complete and how confident the record is.

Record quality

ComprehensiveHigh confidenceNeeds reviewCurated: manual

Literature reviewed 2026-04-26 · schema v1.2.0 · updated 2026-04-28 · imported 2026-04-28

Open data gaps (2)

electrochemical_performance.max_current_density (audit: REQUIRED-missing for electrogen class)
electrochemical_performance.max_power_density (audit: REQUIRED-missing for electrogen class)

References (2)

Lovley DR, et al. (1993). Geobacter metallireducens gen. nov. sp. nov. Arch Microbiol 159:336-344.
Holmes DE, et al. (2017). Metatranscriptomic evidence for DIET between Geobacter and Methanothrix in rice paddy soils. Appl Environ Microbiol 83:e00223-17.doi:10.1128/AEM.00223-17

Version history

2026-04-27 · v1.2.0 · messai_curation
- Added genome_metadata, isolation provenance, type-strain confirmation.
- Added DIET conductive_mineral_mediation evidence (magnetite, Liu 2015).
- Did NOT promote partial → comprehensive: audit flags required electrochemical_performance fields as missing. Promotion deferred until citation-supported values are added.