TEM — Thin section of a recombinant PHB-producing A. woodii cell after 72 h of heterotrophic growth; the white inclusions are engineered polyhydroxybutyrate granules, not a wild-type feature. Species-level image (Acetobacterium woodii [p83_PHB_Scaceti] (recombinant derivative of DSM 1030)). Adapted from Höfele F, Dürre P (2023), Fermentation 9(9):799, Fig. 4A. Source · CC BY 4.0 · Cropped to the high-magnification (right) image of panel A; converted to WebP

Acetogen · Homoacetogen

Acetobacterium woodii

Bacillota · Clostridia · Eubacteriaceae

Type strainBSL-1Partial record
  • Acetogen
  • Negative control MES
  • Sodium atpase
  • Tier 2
  • Important negative example
None
Electron transfer
not electroactive
30°C
Optimal temperature
grows 20–35 °C
7
Optimal pH
grows pH 6–7.8

01 · Identity

Identity

Where this organism sits in the tree of life, how to obtain it, and what a cell looks like.

Lineage

  1. domainBacteria
  2. ›phylumBacillota
  3. ›classClostridia
  4. ›orderEubacteriales
  5. ›familyEubacteriaceae
  6. ›genusAcetobacterium
  7. ›speciesAcetobacterium woodii

Culture collections & accessions

DSMZ
DSM 1030
NCBI taxon
33952
Status
Type strain

Cell morphology

Rod, Gram-positive (monoderm)

  • Motile: no
  • Spore-forming: no

02 · Growth envelope

Growth envelope

The conditions this organism tolerates and what it eats and breathes — the operating window for a reactor.

MesophileObligate anaerobeMixotroph
Temperature30 (20–35) °C
0100 °C
pH7 (6–7.8)
014

Energy metabolism

Electron donors

  • H2
  • Fructose
  • Lactate

Electron acceptors

  • CO2

Carbon sources

  • CO2
  • H2
  • Fructose
  • Lactate
  • Ethanol
  • Methanol
  • Fermentative: yes

Metabolism — detail

ATP synthase type
Sodium dependent
Carbon fixation pathway
Wood ljungdahl
Energy conservation mechanism
Rnf complex

03 · Growth & electron-transfer kinetics

Growth & electron-transfer kinetics

How fast this organism grows and how hard it pushes electrons into an anode, as the corpus reports it, and what those constants imply when you run them.

Kinetic evidence

ParameterMedian10–90 %nSource
Max. specific growth rate μmax0.1 h⁻¹0.05–0.22genus prior · 1 ref
Half-saturation constant Ks30 mg/L5–1001genus prior · 1 ref
Biomass yield Y0.07 g/g0.03–0.121genus prior · 1 ref
Decay coefficient kdNot in the corpus yet
Exchange current density i₀Not in the corpus yet
Transfer coefficient αNot in the corpus yet
Tafel slopeNot in the corpus yet

Median of the extracted values, the 10–90 % spread behind it and how many values it pools.

Evidence rows (0)

Genus-level prior: Acetobacterium woodii

A genus-level prior is a summary for the whole genus, not a measurement on this strain.

Constants that drive the simulation

Scenario — values you set, not evidence

Initial substrate1000 mg/L
Anode potential vs SHE0 V
Duration72 h
Initial attached biomass0.1 g/m²
Anode area per liquid volume100 m²/m³
Reactor mode

Growth simulation

Scenario: Initial substrate 1000 mg/L · Initial attached biomass 0.1 g/m² · Anode area per liquid volume 100 m²/m³ · Duration 72 h · Batch. Values you set, not evidence.

0.8g/m²
Peak biomass
21.15h
Time to 90 % substrate removal
Attached biomassg/m²
00.50204060h
Bulk substratemg/L
050010000204060h

No current model

The current needs Max. current density jmax, Half-saturation potential E_KA, which this source does not have.

  • Substrate-saturated growth
  • Slowing
  • Stationary

Monod growth has no lag phase; the bands mark where the net growth rate is near its maximum, falling, near zero or negative.

Simulator notes
  • decayPerH absent: no endogenous decay term, so growth is an upper bound
  • biofilmDensityKgPerM3 absent: thickness not computed (thicknessUm = null)
  • no anodePotentialVvsShe / overpotentialV given: current not computed (currentDensityAPerM2 = null)

These curves are a Monod and Nernst-Monod idealisation (after Marcus et al. 2007 and Torres et al. 2008), parameterised only by the constants listed above: each one carries its n and its 10–90 % range, and a constant with n = 1 has no spread at all. No diffusion inside the biofilm, no detachment, no coulombic-efficiency split. The scenario values are yours, not evidence.

04 · Performance

Performance

Reported electrochemical output, the role it plays in a reactor, and what it produces.

Substrate → product (1)

  • H2 + CO2yields
    • Acetate

    anaerobic, autotrophic

05 · Ecology & biofilm

Ecology & biofilm

How it lives on an electrode, who it partners with, and where it is found in nature.

Ecosystems

  • Marine
  • Freshwater
  • Anaerobic digester

Isolation sources

  • marine sediment, freshwater sediment

06 · Applications, engineering & safety

Applications, engineering & safety

Where it has been put to work, how tractable it is to engineer, and what handling it requires.

Applications (1)

  • Biofuels
    TRL 4/9

Engineering

Engineered strains publishedIndustrial relevance 3/5

Reactor compatibility

  • MES cathode

Genetics

  • Genetically tractable: yes

Biosafety

BSL-1
  • Pathogen: no

Modeling assets

Linked GEM: iAW773

07 · Sources & data quality

Sources & data quality

Every value above traces to a citation. This is how complete and how confident the record is.

Record quality

PartialHigh confidenceCurated: manual

Literature reviewed 2026-04-26 · schema v1.2.0 · updated 2026-04-28 · imported 2026-04-28

Open data gaps (1)

  • Detailed kinetics under MES conditions

References (1)

  1. Nevin KP, et al. (2011). Electrosynthesis of organic compounds from CO2. AEM 77:2882-2886. — A. woodii notably FAILED to perform MES.doi:10.1128/AEM.02642-10

Version history

  1. 2026-04-27 · v1.2.0 · messai_curation
    • Added energy_conservation_mechanism: rnf_complex (consistent with Na+-translocating ATPase that prevents MES viability).